Table 4.

Comparison of online and offline 3D-LC strategies.

Feature	Offline 3D-LC	Online 3D-LC
Setup	Simple	Usually sophisticated
Ability to transfer technologies to other laboratories	High	Low-medium
Sample amount	High, up to several mg Not suitable for samples with a limited amount	Low, from several µg to 100 µg Allow only low-amount samples
Operation	Separated dimensions, simple and flexible to operate	Usually full-automation, well-trained skill required
Sample pooling	Allow	Usually not allow
Sample handling	High	Low
Sample loss	High-medium	Low