Figure 4.

Automatic/semiautomatic scoring by microscope aided systems (Metasystems). (1) Nuclei of cytokinesis-block cells are visible. System recognizes binucleated cells when both nuclei are the same size and they are in proximity to each other. (2) Motorized microscope with image analysis system safe galleries of binucleated cells and recognize Mn in an automatic manner. (3) Gallery of binucleated cells. Mathematical algorithms to recognize binucleated cells implemented in Metasystems has been used many times with good results [55,115,116]. (4) Each cell from the gallery can be localized and e.g., false positive Mn can be verified in high magnification. Such semiautomatic scoring gives better results than automatic scoring.