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. 2020 Mar 12;8:e8721. doi: 10.7717/peerj.8721

Figure 2. IL-4-induced TAM-like PBM-derived macrophages can secrete CXCL8.

Figure 2

(A–C) Morphological changes of THP-1. PMA (200 nM, 24 h) and IL-4 (20 ng/mL, 48 h) sequentially induce the formation of TAMs-like PBM-derived macrophages. Prior to treatment, THP-1 cells were round, floating, and did not attach to the bottom surface of the flask; after treatment with PMA and IL-4, they adhered to the bottom surface with abundant cytoplasmic processes. (D) After M0 induction using different concentrations of IL-4, the concentration of CXCL8 in serum-free medium was measured over time by ELISA. **p < 0.01, ***p < 0.001. (E) When the concentration of IL-4 was 20 ng/mL, the amount of CXCL8 secreted by TAMs-like PBM-derived macrophages was the greatest. (F) CXCL8 mRNA expression level was determined by qRT-PCR. (G) The expression level of CD163 in TAM-like PBM-derived macrophages. Expression levels are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.