Table 1. Results for brown trout larvae exposed to citalopram.
Temperature | 11 °C | 7 °C | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Treatment (µg/L) | 0 | 1 | 10 | 100 | 1000 | 0 | 1 | 10 | 100 | 1000 |
Mortality (%) | 26.67 ± 2.72 | 32.22 ± 1.57 | 28.89 ± 8.75 | 35.56 ± 4.16 | 22.22 ± 3.14 | 2.22 ± 3.14 | 4.44 ± 4.16 | 3.37 ± 2.72 | 6.74 ± 2.72 | 6.67 ± 2.72 |
Weight (g) | 0.449 ± 0.139 | 0.484 ± 0.169 | 0.447 ± 0.140 | 0.487 ± 0.143 | 0.306 ± 0.146 *** | 0.327 ± 0.089 | 0.413 ± 0.113 | 0.335 ± 0.101 | 0.337 ± 0.091 | 0.247 ± 0.066 *** |
Length (cm) | 3.76 ± 0.37 | 3.83 ± 0.42 | 3.73 ± 0.39 | 3.74 ± 0.40 | 3.24 ± 0.43 *** | 3.33 ± 0.27 | 3.38 ± 0.32 | 3.30 ± 0.32 | 3.25 ± 0.28 | 2.91 ± 0.23 *** |
Heart rate (bpm) | 76 ± 3.74 | n.a. | n.a. | n.a. | 75 ± 4.52 | 49.93 ± 1.5 | 51.8 ± 3.19 | 49.2 ± 3.25 | 50 ± 3.03 | 49 ± 2.37 |
Time to hatch (dpf) | 49.69 ± 0.93 | 49.64 ± 1.11 | 49.74 ± 1.02 | 49.22 ± 0.96 | 49.14 ± 0.84 | 59.38 ± 1.27 | 58.68 ± 1.65 | 59.07 ± 1.31 | 58.60 ± 1.31 | 59.38 ± 1.17 |
Aquaria photographed | 2 | n.a. | 2 | 2 | 2 | 2 | 1 | 2 | 1 | 2 |
Fish in upper aquaria half (%) | 7.29 ± 11.11 | n.a. | 5.71 ± 10.36 | 10.68 ± 13.24 | 66.52 ± 11.28 *** | 0.14 ± 0.68 | 2.3 ± 3.73 * | 2.65 ± 3.22 * | 1.30 ± 2.47 | 79.36 ± 7.19 *** |
ASMD: total distance moved (cm) | 2700 ± 1405 | 1987 ± 755 | 2501 ± 815 | 2252 ± 1353 | 1133 ± 1015 *** | 2473 ± 1016 | 1591 ± 1045 | 1792 ± 1320 | 1361 ± 1033 * | 568 ± 697 *** |
Aqueous citalopram concentration (µg/L) | <LoD | 0.97 ± 0.20 | 8.30 ± 1.17 | 65.74 ± 5.77 | 973.98 ± 180.64 | <LoD | 0.83 ± 0.27 | 8.74 ± 0.48 | 70.50 ± 11.11 | 1017.97 ± 125.84 |
Tissue citalopram concentration (µg/g) (wet weight) | <LoD | 0.07 ± 0.014 | 0.69 ± 0.1 | 1.57 ± 0.451 | 55.87 ± 12.972 | <LoD | 0.2 ± 0.042 | 0.97 ± 0.235 | 5.63 ± 2.0 | 142.15 ± 44.961 |
Notes.
Data are shown as arithmetical means ± standard deviation.
Asterisks represent significant differences to the respective control (*p < 0.05; **p < 0.01; ***p < 0.001).
Abbreviations
- n.a.
- not assessed
- dpf
- day post-fertilisation
- LoD
- limit of detection