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. 2020 Mar 12;8:e8765. doi: 10.7717/peerj.8765

Table 1. Results for brown trout larvae exposed to citalopram.

Temperature 11 °C 7 °C
Treatment (µg/L) 0 1 10 100 1000 0 1 10 100 1000
Mortality (%) 26.67 ± 2.72 32.22 ± 1.57 28.89 ± 8.75 35.56 ± 4.16 22.22 ± 3.14 2.22 ± 3.14 4.44 ± 4.16 3.37 ± 2.72 6.74 ± 2.72 6.67 ± 2.72
Weight (g) 0.449 ± 0.139 0.484 ± 0.169 0.447 ± 0.140 0.487 ± 0.143 0.306 ± 0.146 *** 0.327 ± 0.089 0.413 ± 0.113 0.335 ± 0.101 0.337 ± 0.091 0.247 ± 0.066 ***
Length (cm) 3.76 ± 0.37 3.83 ± 0.42 3.73 ± 0.39 3.74 ± 0.40 3.24 ± 0.43 *** 3.33 ± 0.27 3.38 ± 0.32 3.30 ± 0.32 3.25 ± 0.28 2.91 ± 0.23 ***
Heart rate (bpm) 76 ± 3.74 n.a. n.a. n.a. 75 ± 4.52 49.93 ± 1.5 51.8 ± 3.19 49.2 ± 3.25 50 ± 3.03 49 ± 2.37
Time to hatch (dpf) 49.69 ± 0.93 49.64 ± 1.11 49.74 ± 1.02 49.22 ± 0.96 49.14 ± 0.84 59.38 ± 1.27 58.68 ± 1.65 59.07 ± 1.31 58.60 ± 1.31 59.38 ± 1.17
Aquaria photographed 2 n.a. 2 2 2 2 1 2 1 2
Fish in upper aquaria half (%) 7.29 ± 11.11 n.a. 5.71 ± 10.36 10.68 ± 13.24 66.52 ± 11.28 *** 0.14 ± 0.68 2.3 ± 3.73 * 2.65 ± 3.22 * 1.30 ± 2.47 79.36 ± 7.19 ***
ASMD: total distance moved (cm) 2700 ± 1405 1987 ± 755 2501 ± 815 2252 ± 1353 1133 ± 1015 *** 2473 ± 1016 1591 ± 1045 1792 ± 1320 1361 ± 1033 * 568 ± 697 ***
Aqueous citalopram concentration (µg/L) <LoD 0.97 ± 0.20 8.30 ± 1.17 65.74 ± 5.77 973.98 ± 180.64 <LoD 0.83 ± 0.27 8.74 ± 0.48 70.50 ± 11.11 1017.97 ± 125.84
Tissue citalopram concentration (µg/g) (wet weight) <LoD 0.07 ± 0.014 0.69 ± 0.1 1.57 ± 0.451 55.87 ± 12.972 <LoD 0.2 ± 0.042 0.97 ± 0.235 5.63 ± 2.0 142.15 ± 44.961

Notes.

Data are shown as arithmetical means ± standard deviation.

Asterisks represent significant differences to the respective control (*p < 0.05; **p < 0.01; ***p < 0.001).

Abbreviations

n.a.
not assessed
dpf
day post-fertilisation
LoD
limit of detection