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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Methods Mol Biol. 2018;1846:97–129. doi: 10.1007/978-1-4939-8712-2_7

Fig. 10.

Fig. 10

Imaging of valve LECs. (a) Valve stained for Pecam1 to show valve cell shape. Arrow: elongated cell; arrowhead: rounder valve cell. Scale bar: 20 μm. (b) Valve cells stained for Prox1 and podocalyxin to highlight the sinusal free edge of the valve leaflets. Arrowhead: Prox1HIGH cell at the free edge; arrows: increased deposition of podocalyxin at the free edge. Scale bar: 20 μm. (c) Staining for Prox1 and claudin5 to show intercellular junctions (arrowhead) between valve cells. Scale bar: 25 μm. (d) Valve cell proliferation showed by staining for Prox1 and Ki67. White staining: Lama5. Arrowhead: proliferating Ki67+/Prox1HIGH valve cell; arrow: proliferating Ki67+/Prox1LOW lymphangion cell; asterisk: Ki67+/Prox1neg proliferating non-LEC. Scale bar: 20 μm