Skip to main content
. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Methods Mol Biol. 2018;1846:97–129. doi: 10.1007/978-1-4939-8712-2_7

Table 2.

Examples of applications and criteria of fluorescent microscopy imaging of mesenteric lymphatic valves. Illustrations are shown in Figs. 9 and 10. n.a. not applicable (High-magnification imaging of valves necessitates confocal microscopy, due to their multilayered organization.)

Imaging techniques and AIMS Microscope
Epifluorescence Confocal fluorescence
Magnification (objective) 10–20×

  • Number of valves per vessel length

  • Valve localization within vessel

  • Number of valves per vessel length

  • Valve localization within vessel
20–40×

  • Valve size

  • Valve coverage (mural cells, basement membrane)

  • Leaflet length

  • Leaflet core matrix

  • Cellular organization within the valve (cell shape, cell neighbors, intercellular spreading)
>40× n.a.

  • Valve cell number

  • Valve cell phenotype (identity, shape, junctions, cytoskeleton)

  • Valve buttress

  • Valve cell event (e.g., proliferation)