Fig. 6.

Effects of oxHDL and adiponectin on the secretion of bone associated protein. A: ALP and osteopontin; B: type 1 collagen; C: osteocalcin. About 3x10⁵ cells/well were treated for 24 h with β-glycerophosphate (positive control), media (un-stimulated, negative control), 100 μg/ml HDL, 100 μg/ml oxHDL, 5, 10 and 15 μg/ml adiponectin, and combination of 100 μg/ml oxHDL with 5, 10 and 15 μg/ml adiponectin. The cytosolic protein was extracted using AllPrep® RNA/Protein kit (Qiagen) and was quantified by using specific ELISA kit. The results are shown as mean SEM of 3 experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and **** P≤ 0.0001. *indicates the comparison with oxHDL group, ^#indicates the comparison with unstimulated group