Figure 1: cfDNA 5-hmC profiles are associated with disease burden and disease status in both the Discovery and Validation cohorts.
A) Hierarchical clustering of the Discovery cohort using 347 genes identified in the Discovery cohort with differential 5-hmC between samples from those with or without active disease. B) Hierarchical clustering of the Validation cohort using the same gene set and clustering parameters. Cluster 1 is enriched for patients with high metastatic disease burden. Clusters 2 and 3 are enriched for patients with moderate to high metastatic disease burden. Cluster 4 is enriched for patients with low or no metastatic disease burden.