A) Western blot of ER levels in isolated nuclear (N) and cytoplasmic (C) fractions, after 1-hour treatments with vehicle (DMSO), or 10nM E2. Tubulin and lamin are shown as cytoplasmic and nuclear controls, respectively. B) Relative ratios of nuclear to cytoplasmic ER of duplicate experiments, normalized to the wildtype vehicle ratio, are shown. Error bars represent s.e.m. * = p-value < 0.05, ** indicates p-value < 0.005.