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. 2020 Mar 11;13:2203–2213. doi: 10.2147/OTT.S247015

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© 2020 Wan et al.

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Novel splicing variants of Smad4 expressed during TGF-β-induced EMT in A549 cells. (A) Schematic drawing shows the primer pairs used for NEST-PCR. (B) Representative image of RT-PCR to show the variant expression of Smad4 in A549 cells with or without TGF-β treatment as indicated. More than 3 times, independent experiment repeats were done. (C) Schematic drawing to show the structure of Smad4 and its variants based on sequencing results.