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Microscopic sections of the patients from both families showing marked cerebellar atrophy with thinning of the folia (H&E/LFB low magnification) and Purkinje cell (PC) loss (H&E/LFB high magnification). The loss of PCs is also highlighted by the empty baskets noted on Bielschowsky silver stain and by the severe reduction in calbindin-positive fibers (calbindin and GFAP immunostaining available on request). H&E = hematoxylin and eosin.
