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. 2020 Feb 9;39(6):e103367. doi: 10.15252/embj.2019103367

Figure 2. Excessive excision of incorporated nucleotides during rolling circle DNA synthesis is a general feature of replicative DNAPs.

Figure 2

  1. Experimental design to simultaneously measure Pol‐activity and Exo‐activity during rolling circle DNA synthesis. Reactions with T7 DNAP were performed as described in Fig 1A. Reactions with Mip1 were carried out in the presence of mitochondrial SSB (Rim1), and Φ29 DNAP reactions did not contain any SSB protein. Reactions were performed with 150 μM dNTPs (150 μM dVTPs and 500 μM dTTP in case of T7 DNAP).
  2. Nucleotides incorporated (left panels) and excised (right panels) during rolling circle DNA synthesis by T7 DNAP, Mip1, and Φ29 DNAP. Y‐axes represent moles of nucleotides incorporated or excised per mole of the DNA substrate used.
  3. Pol/Exo ratio determined from the slopes of linear fits of data presented in (B). Pol/Exo‐data for T7 DNAP from Fig 1E (150 μM dVTPs) are reused here for comparison.
Data information: Circles represent data from two individual experiments. Bars show data derived from two individual experiments.