Skip to main content
. 2020 Feb 19;39(6):e102513. doi: 10.15252/embj.2019102513

Figure EV2. Dicer is present in RGC axonal compartment.

Figure EV2

  1. Schematic of FLAG‐HA2‐Dicer. Red arrows indicate primers used for the genotyping spanning the FLAG‐HA2 region. WT, wild type; HA, (FLAG‐HA2)‐Dicer.
  2. Genotyping PCR. WT, wild type; HA, (FLAG‐HA2)‐Dicer; P0, postnatal day 0; E13.5, embryonic day 13.5.
  3. Representative E13.5 retina stained for anti‐HA antibody. Dashed light blue box indicates the region zoomed. WT, wild type; E13.5, embryonic day 13.5. Scale bars: 50 μm.
  4. Representative P0 superior colliculus stained with anti‐neurofilament and anti‐HA antibodies to detect RGC axons in the stratum griseum superficiale of the superior colliculus and (FLAG‐HA2)‐Dicer, respectively. Dashed white box indicates the region zoomed. Zoom of the triple‐stained P0 superior colliculus in a nucleus‐free region. WT, wild type; P0, postnatal day 0. Scale bars: 30 μm.

Source data are available online for this figure.