Figure EV4. Morpholino mix validation.

• A
  Schematic representation of the experimental paradigm. co‐MO, control morpholino.
• B, C
  Quantification of the expression levels of miRNAs using the 2^−ΔCt method and U6 as normalizer. Concentrations used are as follows: 150–200 μM co‐MO or MOs‐5p or MOs‐3p. Data are presented normalized to co‐MO. Each data point corresponds to one independent experiment. n = 4 independent experiments. Values are mean ± SEM. co‐MO, control morpholino.
• D
  Schematic representation of the experimental paradigm. co‐MO, control morpholino.
• E–G
  Quantification of the expression levels of miR‐181a‐5p (E, G) and miR‐181a‐1‐3p (F) and using the 2^−ΔCt method and U6. Concentrations used are as follows: 2 μM MOs‐5p or MOs‐3p or co‐MO, 200 ng/ml Sema3A or Slit‐2. Data are presented normalized to PBS control. Each data point represents a single RT–qPCR. n = 4 (E, G) and n = 6 (F) independent experiments. Values are mean ± SEM. ns, not significant

Data information: *P < 0.05. Data were not normally distributed (Shapiro–Wilk test). Two‐tailed Mann–Whitney test (B, C, E, F, G).