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. 2020 Mar 4;2020:5135893. doi: 10.1155/2020/5135893

Figure 3.

Figure 3

UCP2 knockdown increases apoptosis of irradiated HeLa cells. (a) Flow cytometry analysis of apoptosis after UCP2 knockdown with or without irradiation. (b) Western blots of Bcl-2, Bcl-xl, caspase-3, caspase-9, cytochrome c, Apaf-1, and the β-actin protein loading control. (c) Relative protein amount of Bcl-2, Bcl-xl, caspase-3, caspase-9, cytochrome c, and Apaf-1. Bars are means ± SD of triplicate assays. ∗P < 0.05 and ∗∗P < 0.01 vs. control, #P < 0.05 and ##P < 0.01 vs. 4 Gy irradiation.