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UCP2 knockdown and cell cycle arrest of irradiated cancer cells in G2/M. (a) Flow cytometry of HeLa cells after UCP2 knockdown with or without irradiation. (b) Western blots of cyclin B, CDC2, P53, and the β-actin loading control expression. (c) Relative protein amount of cyclin B, CDC2, and P53. Bars and data are means ± SD of triplicate assays. ∗P < 0.05 and ∗∗P < 0.01 versus control, #P < 0.05 and ##P < 0.01 vs. 4 Gy irradiation.
