Figure 3.

Invasion and migration ability of LIF in OSCC cells. (A,B) Western blot analysis of LIF expression in CA9-22 or SAS cells transiently transfected with LIF-expressed or shLIF plasmid. β-actin was used as an internal control. An in vitro migration and invasion assay was used to evaluate cell migration and invasion ability, performed for 24 or 48 h (* p <0.05, ** p < 0.01). (C,D) CA9-22 and SAS cells were subcultured in a Boyden chamber and treated with various concentrations of recombinant LIF protein (rLIF) or LIF neutralization antibody overnight. Cell motility toward the lower face of the filter was observed and quantified (* p < 0.05, ** p < 0.01).