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Separation of 2,3-dehydrosilybin enantiomers using chiral column Lux 3μ Cellulose-4 (2 × 50 mm) and pre-column (4 × 2 mm) and binary gradient elution with mobile phase A: 10% acetonitrile, 0.1% formic acid, and B: 80% acetonitrile, 0.1% formic acid; gradient: 0 min 30% B, 0–12 min 30–50% B, 12–13 min 50–30% B, 13–15 min 30% B; flow rate 0.5 mL/min, t = 25 °C.
