Figure 3.

Differences in telomere parameters between normal breast cells, p53 knockout and isogenic wild-type MCF-7 cells. (A) The total number of telomere signals. (B) The total number of telomere aggregates (telomeres in close proximity that cannot be further resolved at an optical resolution limit of 200 nm). (C) Total telomere signal intensity (proportional of telomere length). (D) a/c ratio (nuclear spatial distribution of telomeres). The a/c ratio is defined as the nuclear space occupied by telomeres, represented by three axes of length a, b and c. The ratio between the a and c axes, the a/c ratio, reflects the distribution of telomeres, which changes at different stages of the cell cycle. (E) p-values for each comparison. Std Dev—standard deviation; H0—null hypothesis.