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. 2020 Mar 15;8(1):e000465. doi: 10.1136/jitc-2019-000465

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Necrotic phenotype accompanies triple-negative breast cancer (TNBC) cell death following infection with VSVd51. (A) Light microscopy images of TNBC cell lines infected with 10 multiplicity of infection (MOI) of VSVd51 for 24 hours. (B) Electron microscopy images of TNBC cell lines infected with 10 MOI of VSVd51 for 72 hours. (C) Cell viability assay, (D) Western blot analysis of HMGB1 from cell-free supernatants, (E) luminometry measurement of relative ATP from cell-free supernatants and (F) measurement of cell surface calreticulin of TNBC cell lines infected with VSVd51 at indicated MOI and following indicated time points. All data are representative of at least three similar experiments where n=3 for technical replicates, *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; n.s., not significant.