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. 2020 Mar 15;8(1):e000465. doi: 10.1136/jitc-2019-000465

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Infected cell vaccine (ICV) enhances immune signature and biomarkers of immunogenic cell death in human triple-negative breast cancer (TNBC) patient tissue. Fold change in gene expression from human TNBC patient tissue (A) BRC1762 and (B) BRC1756 following infection with VSVd51 at 10 multiplicity of infection (MOI) for 24 hours. (C) Measurements of cell surface calreticulin, (D) luminometry measurement of relative ATP and (E) Western blot analysis of HMGB1 from cell-free supernatants from TNBC patient tissue following infection with VSVd51 after 24 hours and at indicated MOI. Data are pooled from technical replicates, n=3, *p<0.05; **p<0.01; n.s., not significant.