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. 2019 May 24;112(3):266–277. doi: 10.1093/jnci/djz097

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Figure 2. — Effects of ΔNp63α levels on microRNA (miR)-27a* expression in head and neck squamous cell carcinoma (HNSCC) cells. ΔNp63α was depleted using a ΔNp63-targeting small interfering RNA (siRNA) in the indicated HNSCC cell lines. Expression of miR-27a* in (A) JHU-029, (B) HN31, and (C) UM-SCC-17A (17A) cells and ΔNp63 mRNA in (D) JHU-029, (E) HN31, and (F) 17A cells were analyzed by quantitative real-time polymerase chain reaction (qPCR). Error bars represent mean (SEM). Protein levels were analyzed by immunoblotting in (G) JHU-029, (H) HN31, and (I) 17A cells treated with either control (Con) or ΔNp63 siRNA with β-actin as an endogenous control. Endogenous controls used in qPCR analysis were RNU44/RNU6B for miRNA and glyceraldehyde-3-phosphate dehydrogenase for mRNA.