Figure 5.

Antitumor activity of AspA in a nude mouse xenograft model. (A) RKO xenograft-bearing nude mice were treated intraperitoneally with the indicated drugs three times per week for 21 days (n = 5 per group). For combination, 4 mg/kg of AspA and 4 mg/kg of irinotecan were administered. Tumor volumes were measured every 3–4 days. (B) Ki67 protein expression in RKO cell xenograft tumors was determined by immunohistochemical analysis. Scale bar, 100 μm. (C) The bodyweight of mice bearing RKO xenografts was measured every 3–4 days during the treatment with the indicated drugs (n = 5 per group). The error bars represent the means ± SD. * P < 0.05, ** P < 0.01, *** P < 0.005 by t-test.