Table 1.
Phosphatidylcholine (PC) species in pork chop detected by LC–electrospray ionization (ESI)–MS/MS.
| [M + H]+, m/z | Molecular Species | Relative Intensity (%) | Product ions for Assignment, m/z |
|---|---|---|---|
| 732.5 | (diacyl 16:0/16:1) | 1.20 ± 0.08 | 184, 476, 478 |
| 734.5 | (diacyl 16:0/16:0) | 1.08 ± 0.07 | 184, 478 |
| 758.5 | (diacyl 16:0/18:2) | 27.13 ± 0.96 | 184, 478, 502 |
| 760.5 | (diacyl 16:0/18:1) | 16.77 ± 0.51 | 184, 478, 504 |
| 782.6 | (diacyl 16:0/20:4) | 0.88 ± 0.07 | 184, 478, 526 |
| 784.6 | (diacyl 18:1/18:2) | 2.53 ± 0.07 | 184, 502, 504 |
| 786.6 | (diacyl 18:0/18:2) (diacyl 18:1/18:1) |
5.04 ± 0.09 2.34 ± 0.05 |
184, 502, 506 184, 504 |
| 788.6 | (diacyl 18:0/18:1) | 2.67 ± 0.10 | 184, 504, 506 |
| 808.6 | (diacyl 18:1/20:4) | 0.28 ± 0.03 | 184, 504, 526 |
| 810.6 | (diacyl 18:0/20:4) | 0.43 ± 0.01 | 184, 506, 526 |
| Total | 60.34 | ||
| 720.5 | (alkylacyl 16:0/16:0) | 0.48 ± 0.02 | 184, 482, 478 |
| 742.5 | (alkenylacyl 16:0/18:2) | 8.67 ± 0.21 | 184, 480, 502 |
| 744.5 | (alkylacyl 16:0/18:2) (alkenylacyl 16:0/18:1) |
11.40 ± 0.29 1.40 ± 0.06 |
184, 482, 502 184, 480, 504 |
| 746.5 | (alkylacyl 16:0/18:1) | 3.88 ± 0.20 | 184, 482, 504 |
| 766.6 | (alkenylacyl 16:0/20:4) | 1.50 ± 0.04 | 184, 480, 526 |
| 768.6 | (alkylacyl 16:0/20:4) (alkenylacyl 18:1/18:2) |
2.20 ± 0.12 2.13 ± 0.03 |
184, 482, 526 184, 506, 502 |
| 770.6 | (alkylacyl 18:1/18:2) | 3.59 ± 0.18 | 184, 502, 508 |
| 772.6 | (alkylacyl 18:0/18:2) (alkylacyl 18:1/18:1) (alkenylacyl 18:0/18:1) |
0.86 ± 0.04 0.97 ± 0.03 1.15 ± 0.01 |
184, 510, 502 184, 508, 502 184, 508, 504 |
| 794.6 | (alkenylacyl 18:0/20:4) | 1.43 ± 0.01 | 184, 508, 526 |
| Total | 39.66 |
The total lipid extracts from the pork chop sections were subjected to precursor ion scanning at m/z 184.1 to detect phosphatidylcholine (PC) species. Product ion scanning was used for the analysis of fatty acid composition. PC species were assigned based on product ions and previous reports [4,13,14]. The relative intensities of identified PC species are presented as their average ± standard deviation (n = 3).