Table 5.
Canonical pathways of differentially expressed genes between normal and scrotal hernia-affected pigs.
| Canonical Pathway | Genes | p-Value | |
|---|---|---|---|
| ssc05410 | Hypertrophic cardiomyopathy (HCM) | TGFB3 ; TPM1; ACTC1; TPM2; MYL3; TNNC1; PRKAG3; CACNG1; CACNB1; SGCA; DES; CACNA1S | 4.16 × 10−6 |
| ssc05414 | Dilated cardiomyopathy | TGFB3 ; TPM1; ACTC1; TPM2; ADRB1; MYL3; TNNC1; CACNG1; CACNB1; SGCA; DES; CACNA1S | 7.72 × 10−6 |
| ssc04261 | Adrenergic signaling in cardiomyocytes | MYH7; TPM1; ACTC1; TPM2; ATP1A2; PLCB4; ADRA1A; ADRB1; MYL3; TNNC1; SCN7A; CACNG1; CACNB1; CACNA1S | 2.64 × 10−5 |
| ssc04022 | cGMP-PKG signaling pathway | SLC8A3; ATP1A2; PLCB4; MYLK2; ATP2A1; EDNRB; ADRA1A; PPIF; ADRB1; MYLK; SLC25A4; KCNMB1; MRVI1; ADORA1; CACNA1S | 5.02 × 10−5 |
| ssc04260 | Cardiac muscle contraction | MYH7; TPM1; ACTC1; TPM2; ATP1A2; MYL3; TNNC1; CACNG1; CACNB1; CACNA1S | 9.46 × 10−5 |
| ssc04152 | AMPK signaling pathway | LIPE; ADRA1A; FBP2; PFKFB3; PPARG; PRKAG3; PFKM; CPT1B; SLC2A4; LEPR; PPARGC1A | 8.12 × 10−4 |
| ssc04020 | Calcium signaling pathway | SLC8A3; PLCB4; MYLK2; TNNC2; ATP2A1; EDNRB; ADRA1A; PPIF; ADRB1; TNNC1; MYLK; SLC25A4; CACNA1S | 2.04 × 10−3 |
| ssc00250 | Alanine, aspartate and glutamate metabolism | ALDH5A1; GPT2; DDO; ABAT; ASPA | 1.31 × 10−2 |
| ssc04923 | Regulation of lipolysis in adipocytes | LIPE; ADRB1; ENSSSCG00000010992; PNPLA2; ADORA1; MGL | 1.51 × 10−2 |
| ssc01100 | Metabolic pathways | TST; PIK3C2G; ALDH5A1; ANPEP; FUT8; GPT2; AK5; PGM1; GALNT12; AK1; PHGDH; AMPD1; PLCB4; GUSB; ABAT; ST3GAL5; B3GNT2; LPIN1; CMPK2; HPGDS; HPSE; EPHX2; GAL3ST1; FBP2; ACAA1; PLD1; PNPLA2; PC; PYGM; CKMT2; P4HA3; CYP27A1; ATP6V0A4; PGAM2; ASPA; ENO3; PFKM; ; CKB; DGKG; PYGB; MGLL; ACSM5 | 1.81 × 10−2 |
| ssc04910 | Insulin signaling pathway | LIPE; FBP2; PYGM; PRKAG3; PPP1R3A; PPP1R3B; PYGB; SLC2A4; PPARGC1A | 1.93 × 10−2 |
| ssc01230 | Biosynthesis of amino acids | GPT2; PHGDH; PC; PGAM2; ENO3; PFKM | 1.98 × 10−2 |
| ssc04931 | Insulin resistance | PYGM; PRKAG3; PPP1R3A; CPT1B; PPP1R3B; PYGB; SLC2A4; PPARGC1A | 2.49 × 10−2 |
| ssc03320 | PPAR signaling pathway | OLR1 ; ENSSSCG00000010992; ACAA1; PPARG; CYP27A1; CPT1B | 2.85 × 10−2 |
| ssc04270 | Vascular smooth muscle contraction | PLCB4; MYLK2; ADRA1A; ACTA2; MYLK; KCNMB1; MRVI1; CACNA1S | 2.95 × 10−2 |
| ssc04922 | Glucagon signaling pathway | PLCB4; PYGM; PRKAG3; PGAM2; CPT1B; PYGB; PPARGC1A | 3.51 × 10−2 |
| ssc04921 | Oxytocin signaling pathway | PLCB4; MYLK2; MYLK; CAMK1G; PRKAG3; CACNG1; CACNB1; KCNJ5; CACNA1S | 3.91 × 10−2 |
| ssc01200 | Carbon metabolism | GPT2; PHGDH; FBP2; PC; PGAM2; ENO3; PFKM | 4.90 × 10−2 |
Upregulated genes in scrotal hernia-affected pigs are shown in bold.