Table 1.
Primers used in this study.
| Primer Name | Sequence (5′-3′) |
|---|---|
| TmSpz6-qPCR-Fw | GCACAACTCCAAGACGACCT |
| TmSpz6-qPCR-Rv | TCTCTTCACCCGATCGTTGC |
| TmSpz6-T7-Fw | TAATACGACTCACTATAGGGTACCGCGCAAGAGAGTAAAAA |
| TmSpz6-T7-Rv | TAATACGACTCACTATAGGGTACGTATCTCCACACCCCTTG |
| TmSpz6-cloning-Fw | CCCCTGTCGACTGCAAAGAA |
| TmSpz6-cloning-Rv | CACCACGTATCTCCACACCC |
| TmSpz6-cloning-FullORF-Fw | TGAGTGAATAATTTCGAAAAGAAAAA |
| TmSpz6-cloning-FullORF-Rv | TGGGCGTTCAGTTACATCAA |
| TmL27a_qPCR_Fw | TCATCCTGAAGGCAAAGCTCCAGT-3′ |
| TmL27a_qPCR_Rv | AGGTTGGTTAGGCAGGCACCTTTA-3′ |
| dsEGFP_Fw | TAATACGACTCACTATAGGGTCGTAAACGGCCACAAGTTC |
| dsEGFP_Rv | TAATACGACTCACTATAGGGTTGCTCAGGTAGTGTTGTCG |
| TmTencin-1_Fw | CAGCTGAAGAAATCGAACAAGG |
| TmTencin-1_Rv | CAGACCCTCTTTCCGTTACAGT |
| TmTencin-2_Fw | CAGCAAAACGGAGGATGGTC |
| TmTencin-2_Rv | CGTTGAAATCGTGATCTTGTCC |
| TmTencin-3_Fw | GATTTGCTTGATTCTGGTGGTC |
| TmTencin-3_Rv | CTGATGGCCTCCTAAATGTCC |
| TmTencin-4_Fw | GGACATTGAAGATCCAGGAAAG |
| TmTencin-4_Rv | CGGTGTTCCTTATGTAGAGCTG |
| TmDefensin-1_Fw | AAATCGAACAAGGCCAACAC |
| TmDefencin-1_Rv | GCAAATGCAGACCCTCTTTC |
| TmDefencin-2_Fw | GGGATGCCTCATGAAGATGTAG |
| TmDefencin-2_Rv | CCAATGCAAACACATTCGTC |
| TmColoptericin-1_Fw | GGACAGAATGGTGGATGGTC |
| TmColoptericin-1_Rv | CTCCAACATTCCAGGTAGGC-3 |
| TmColoptericin-2_Fw | GGACGGTTCTGATCTTCTTGAT |
| TmColoptericin-2_Rv | CAGCTGTTTGTTTGTTCTCGTC |
| TmAttacin-1a_Fw | GAAACGAAATGGAAGGTGGA |
| TmAttacin-1a_Rv | TGCTTCGGCAGACAATACAG |
| TmAttacin-1b_Fw | GAGCTGTGAATGCAGGACAA |
| TmAttacin-1b_Rv | CCCTCTGATGAAACCTCCAA |
| TmAttacin-2_Fw | AACTGGGATATTCGCACGTC |
| TmAttacin-2_Rv | CCCTCCGAAATGTCTGTTGT-3 |
| TmCecropin-2_Fw | TACTAGCAGCGCCAAAACCT |
| TmCecropin-2_Rv | CTGGAACATTAGGCGGAGAA |
| TmThaumatin-like protein-1_Fw | CTCAAAGGACACGCAGGACT |
| TmThaumatin-like protein-1_Rv | ACTTTGAGCTTCTCGGGACA |
| TmThaumatin-like protein-2_Fw | CCGTCTGGCTAGGAGTTCTG |
| TmThaumatin-like protein-2_Rv | ACTCCTCCAGCTCCGTTACA |
| Underline indicates T7 promoter sequences |