Figure 8.

HPLC-MS Analyses of the Oxidative and Nitrative Modification of Aβ₁₆

(A) Aβ₁₆ incubated under hemin-nitration conditions using heme-H₂O₂-NaNO₂ with varying concentrations of MMn. Percent composition of Aβ₁₆ is shown in black and, as expected, increases with the [MMn].

(B) The protective effect of MMn was also tested against heme-peroxynitrite-mediated modification of Aβ₁₆ in comparison with MnTMPyP. Sodium ascorbate (NaAsc) was added to regenerate the MMn catalyst under the reaction conditions. Reported values are averages with the corresponding standard deviation as obtained from two independent measurements.