Figure 5.

Cell cycle analysis of MG63 cells after 3 days treatment with different fucoidans at 0, 0.5 and 100 μg/mL. Nearly 10,000 single cell events were measured after staining with PI (n = 1). Populations were split into sub-G1, G1, S and G2 phases. Notice the presence of more cells in sub G1, S and G2 phases (green arrows) and fewer cells in G1 phase in after treatment with 100 μg/mL of crude fucoidan from F. vesiculosus relative to other conditions. There were also fewer cells in S and G2 phases and more cells in G1 phase after treatment with 100 μg/mL of MMW or HMW (blue arrows) derived from S. filipendula compared to untreated condition or lower doses of same fucoidans.