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. 2020 Feb 8;18(2):104. doi: 10.3390/md18020104

Figure 8.

Figure 8

Assessment of mitochondria in MG63 cells after 3 days treatment with different fucoidans. (A) Mitochondrial membrane potential changes measured using JC-1 staining (at least 10 cells were analysed per condition). (B) Representative images of live cells taken under 525 nm (green) and 647 nm (red) channels with overlays. Scale bars—25 µm. (C) Mean ± SD of Cyt C signal to DAPI signal (n = 3 fields of view). (D) Representative overlay images of cells stained with Cyt C (Red) and DAPI (blue). Arrows—localization of Cyt c in perinuclear regions. Scale bar—10 µm. * p < 0.05, # p < 0.05 relative to respective vehicle controls.