Figure 7.

IGFBP-1 over expression in IRKO mice which have impaired hind limb ischemia recovery. (A–D) IRKO mice were used as a model of insulin resistance to investigate the effects on hind limb ischemia recovery. (A) Representative laser Doppler blood perfusion images of mouse hind limbs on day 0, 7, 14, and 21 after injury. (B) Quantitative analysis of the perfusion recovery measured by laser Doppler. The index was calculated as the ratio of ischemic to non-ischemic hind limb blood perfusion. There is significant impaired recovery in IRKO mice compared with wild-type litter mate controls at day 7 post surgery (IRKO 19.1% ± 2.3 V Wt 27.8% ± 4.6) and also shown in area under the curve in C (IRKO 77.8 ± 6.1 V Wt 108.6 ± 11 v IRKOxIGFBP-1-Tg 116.2 ± 13.7). IGFBP-1 overexpression improves recovery when compared to IRKO wild-type litter mate controls, as shown in area under the curve (IRKO 77.7 ± 6.1 V IGFBP-1 × IRKO 116.2 ± 13.7). (D) Gastrocnemius from ischemic and nonischemic limbs were stained with isolectin B4 (red) as a marker of endothelial cells and DAPI (blue) as a nuclear stain and capillary density quantified (Wt 17 ± 2 V IRKO 5.8 ± 2.1 V IRKO × IGFBP-1-Tg 15.03 ± 5.3) N = WT 6, IRKO 12, and IGFBP-1 × IRKO 19. Data are presented as mean ± SEM. (*P ≤ .05).