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. 2019 Nov 15;11(3):371–378. doi: 10.1021/acsmedchemlett.9b00445

Figure 8.

Figure 8

CD4mc sensitize HIV-1 infected cells to ADCC. Primary CD4 T cells isolated from PBMC were infected with HIV-1CH58TF for 48 h. For cell surface staining, 5 μg/mL 17b (A) or 1:1000 diluted HIV+ plasma (n = 5) (B) were used in the presence of 50 μM of the different MCG analogs, (+)-BNM-III-170, or with equivalent volume of vehicle (DMSO), and an Alexa Fluor 647-conjugated antihuman IgG secondary Ab was then used for fluorescent labeling. For ADCC (C), infected cells were used as target cells in a FACS-based ADCC assay that measures the killing of infected (p24+ cells) to determine their susceptibility to ADCC mediated by a 1:1000 dilution of plasma from 5 HIV-1-infected individuals in the presence of 50 μM different MCG analogs, (+)-BNM-III-170, or with equivalent volume of vehicle (DMSO). Data shown are the mean ± SD of three independent experiments. Statistical significance was evaluated using paired t test (*, P < 0.05; **, P < 0.01; ***, p < 0.001; ns, not significant).