Figure 6.

Oral ultramicronized palmitoylethanolamide treatment did not affect synaptophysin levels in the hippocampus of non-Tg and 3×Tg-AD mice. Age-matched non-Tg mice and 3×Tg-AD mice (2 months ± 2 weeks of age) have been orally treated for 3 months with the compound (100 mg/kg/day, added to the animal food), while control animals were fed with a standard diet during the treatment period. Representative western blot densitometric analysis of synaptophysin is reported above the graph. Western blot analysis was performed on hippocampus tissues taken from mouse brains at the end of the treatment period. Experiments were performed three times in triplicate and β-Tubulin was used as loading control. Results are expressed as percentage of the mean ± SEM of control value (i.e., standard diet fed non-Tg mice).