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Hemagglutination and inhibition assay. The purified C-terminal half of galectin-3 (galectin-3C) (50 μg protein/ml final concentration) in upper left well was twofold serially diluted and mixed with horse blood (final well concentration 1%), BSA in PBS (final well concentration 0.25%), upper row. C1 is control with PBS instead of galectin-3C. C2 is control with 0.5 M lactose in the presence of 50 µg/mL galectin-3C), showing inhibition of hemagglutination.
