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. 2020 Mar;190(3):711–722. doi: 10.1016/j.ajpath.2019.11.011

Figure 1.

Figure 1

Deficits in alveolarization and lung vascularization of 2-week–old adrenomedullin-deficient (ADM+/−) mice. Lung tissues of mice exposed to 21% oxygen (normoxia) were harvested on postnatal day 14 for lung morphometry and gene expression assays. A and B: Representative hematoxylin and eosin–stained lung sections obtained from adrenomedullin-sufficient (ADM+/+) (A) and ADM+/− (B) mice. C and D: Representative von Willebrand factor (vWF)–immunostained lung sections (arrows) obtained from ADM+/+ (C) and ADM+/− (D) mice. E–G: Alveolarization was quantified by determining radial alveolar counts (E) and mean linear intercepts (F), and pulmonary vascularization was quantified by counting the vWF-stained lung blood vessels (G). H: RT-PCR analyses–based determination of ADM mRNA levels. Data are expressed as means ± SD. n = 5 to 8 mice per group. **P < 0.01, ***P < 0.001 versus ADM+/+ mice (t-test). Scale bars = 100 μm. HPF, high-power field.