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. 2020 Mar;190(3):711–722. doi: 10.1016/j.ajpath.2019.11.011

Figure 6.

Figure 6

Quantification of apoptosis in the lungs of neonatal adrenomedullin-deficient (ADM+/−) mice exposed to hyperoxia. Lung tissues of mice exposed to 21% oxygen (normoxia) or 70% oxygen (hyperoxia) were collected on postnatal day (PND) 7 or PND14 for gene and protein expression assays. A and B: RT-PCR analyses–based determination of BAX (A) and TNFRSF10B (B) mRNA levels. C: Determination of cleaved caspase 3 protein levels by immunoblotting. D: Quantification and normalization of cleaved caspase 3 band intensities to those of β-actin. Data are expressed as means ± SD. n = 3 mice per group. *P < 0.05, ***P < 0.001 versus ADM+/+ mice; ††P < 0.01, †††P < 0.001 versus normoxia.