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. 2020 Mar;190(3):711–722. doi: 10.1016/j.ajpath.2019.11.011

Figure 7.

Figure 7

Quantification of collagen in the lungs of neonatal adrenomedullin-deficient (ADM+/−) mice exposed to hyperoxia. Lung tissues of mice exposed to 21% oxygen (normoxia) or 70% oxygen (hyperoxia) were collected on postnatal day (PND) 14 or PND28 to quantify collagen staining by immunohistochemistry studies (trichrome staining). A–D: Representative lung sections showing collagen staining (blue; arrows) obtained at PND14 from adrenomedullin-sufficient (ADM+/+) (A and C) and ADM+/− (B and D) mice exposed to normoxia (A and B) or neonatal hyperoxia (C and D). E–H: Representative lung sections showing collagen staining (blue; arrows) obtained at PND28 from ADM+/+ (E and G) and ADM+/− (F and H) mice exposed to normoxia (E and F) or neonatal hyperoxia (G and H). I: Quantification of the percent area of collagen staining. Data are expressed as means ± SD. n = 3 mice per group. *P < 0.05, ***P < 0.001 versus ADM+/+ mice; ††P < 0.01, †††P < 0.001 versus normoxia. Scale bars = 100 μm.