Figure 3.

Cell density (log₁₀ (CFU/cm²)) of the 1-day-old L. monocytogenes, S. Typhimurium, and dual-species model biofilms as function of the CAP treatment time using an air-based (black) or helium-operated (red) CAP system (n = 3). Both the experimental data (symbols) and the global fit (line) of the Geeraerd et al. [25] model are represented. For the single-species (A) L. monocytogenes and (B) S. Typhimurium biofilms: total viable population on general medium (o, solid line) and uninjured viable population on selective medium (x, dashed line). For the (C) dual-species biofilm: total population on general medium (o, solid line), L. monocytogenes population on PALCAM medium (x, dash=dot line), and S. Typhimurium population on XLD medium (◊, dashed line). The percentage of sub-lethally injured cells (SI) as function of the CAP treatment time have been included as well for the (D) L. monocytogenes and (E) S. Typhimurium single-species model biofilms using an air-based (black) or helium-operated (red) CAP system.