Supplemental Figure S5.

Effect of galanin on expression of genes associated with hepatic fibrosis in FVBN and Mdr2KO mice. A–D: Expression of α-smooth muscle actin (α-SMA; A), Col1A1 (B), MMP2 (C), and TIMP1 (D) at mRNA level was assessed by real-time quantitative PCR in liver from FVBN and Mdr2KO mice, 2 months old, treated with vehicle (Veh) or galanin. E, G, and I: Liver sections from these mice were stained for desmin (E) and α-SMA (G) by immunohistochemistry and for collagens I and III (I) with Sirius Red. F, H, and J: Quantifications were performed by image analysis. n = 4 (A–D); n = 5 (F,H, and J). *P < 0.05 Gal versus vehicle; ^†P < 0.05 Mdr2KO versus FVBN; ^‡P < 0.05 males versus females. Scale bars = 100 μm (E, G, and I).