T/C-28a2 chondrocytic cell line viability and proliferation (directly proportional to alamar blue dye reduction) after 24, 48 and 72 h incubation in basal medium (Dulbecco’s modified Eagle’s medium (DMEM) 10%) supplemented with different concentrations of CS (10 µg/mL, 50 µg/mL, 100 µg/mL and 200 µg/mL) or CS-free, derived from fish (Prionace glauca, Raja clavata and Scyliorhinus canicula) and bovine sources. Significant statistical differences for p < 0.05 (*) and p < 0.01 (**) are shown. No significant differences were found amongst the different CS sources and CS-free condition.