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Coupling flexivent and hemodynamics does not affect tissue morphology. Lungs were agarose inflated using constant pressure to obtain tissue for histological H&E or trichrome staining. Representative images were presented for young WT mice: (a) Hdx only, (b) flexiVent only, and (c) flexivent + Hdx or flexivent + Hdx for aged (d) WT and (e) and (f). BOE mice. Scale bars = 100 μM. n = 7–9 mice/group.
