Figure 2.

Isolation of naEVs from E4.5 blastocyst spent media. (A) Size distribution and quantification using nanoparticle tracking analysis of naEVs isolated from spent blastocyst media. Net particle concentration was calculated after subtracting particles found in the same volume of blank media. Values represent the mean of three independent experiments ±SEM. (B) Different images showing morphological characterization of naEVs in spent culture media by TEM. Particles of different aspects, electron densities and sizes ranging from 20–500 nm were observed.