Figure 5.

Analysis of murine-specific DNA sequences secreted by the embryo to the culture media. Study of DNA sequences uniquely mapping to Mus musculus reference genome secreted by the murine embryo to the spent media in EVs or as a free form. (A) PCA showing similarity between samples using normalized counts of 27,970 genomic features after filtering out human-homologous reads. (B) PCA grouping samples by DNase treatment. (C) Separate PCA of DNase untreated samples using normalized counts of 27,824 genomic features after filtering out human-homologous reads. (D) Volcano plots showing differential enriched genes in the studied fractions (embryo, ABs, naEVs, or SN) compared to the blank control. Negative FC values (left) indicate genes that are enriched in each fraction with respect to the blank medium, while positive values (right) correspond to genes enriched in the blank medium compared to the studied sample. The number of differentially enriched genes is shown in brackets for each comparison. (E) Venn diagram showing common and specific enriched genes in pairwise comparisons. Abbreviations: AB, apoptotic bodies; naEVs, smaller in size extracellular vesicles (including microvesicles and exosomes); SN, supernatant.