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Validation of on-chip preconcentration and RT-PCR of H1N1 in saliva by gel electrophoresis. Gel electrophoresis of PCR products (M gene) from 1 mL saliva containing influenza A virus H1N1 at different concentration (102–104 TCID50/mL) without preconcentration (a) and on-chip preconcentration and RT-PCR using µFPNAS (b). (c,d) Analysis of intensity levels for the red rectangular areas in (a,b), respectively.
