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. 2020 Jan 23;8(2):164. doi: 10.3390/microorganisms8020164

Figure 2.

Figure 2

Complementation studies with Mbov_0503 knock-out mutant. (A) PCR amplification of Mbov_0503 in the parental strain (HB0801), Mbov_0503 knock-out mutant (T4.4), and complemented strain (CPT4.4), (B) RT-PCR amplification of Mbov_0503 transcripts, (C) electrophoretic analysis of total RNA, and (D) mycoplasma binding to EBL cells. Data are the means of three independent assays. Standard deviations are indicated by error bars. P values are indicated by asterisks (** p < 0.01; ns = p > 0.05). (E) Immunodetection of Mbov_0503 products at the mycoplasma surface. Colonies were incubated with rMbovP0503 or rVspX antisera.