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Immunoreactivity of rMbovP0503. (A) SDS-PAGE analysis of purified rMbovP0503. (B) Western blotting analysis of rMbovP0503 reactivity with the rMbovP0503 antiserum, (C) cattle antiserum against HB0801, and (D) negative cattle serum. The unrelated M. bovis recombinant protein rMbovP328 was used as a negative control. M: prestained protein ladder.
