Table 2.
Transposon insertion sites in M. bovis mutants with reduced binding to EBL cells
| Genomic Position a | CDS b | Relative CDS Position c | Mutant d | Predicted CDS Identity |
|---|---|---|---|---|
| 035818 (-) | Mbov_0034 | 0.07 (+) | T5.407 | oligopeptide ABC transporter ATP-binding protein (oppD) |
| 068606 (+) | Mbov_0059 | 0.99 (-) | T5.182 | tRNA modification GTPase (trmE) |
| 175200 (-) | Mbov_0154 | 0.61 (-) | T5.187 | transmembrane protein |
| 191067 (+) | Mbov_0168 | 0.31 (-) | T5.201 | trigger factor (tig) |
| 359714 (-) | Mbov_0305 | 0.41 (+) | T5.230 | transmembrane protein |
| 420981 (+) | Mbov_0353 | 0.45 (+) | T5.212 | alcohol dehydrogenase (adh) |
| 436842 (+) | Mbov_0370 | 0.38 (+) | T5.313 | tRNA-methyltransferase (trmU) |
| 573330 (+) | Mbov_0490 | 0.97 (+) | T5.495 | ATP-binding cassette subfamily B |
| 586897 (-) | Mbov_0503 | 0.23 (-) | T4.4 | transmembrane protein |
a Transposon insertion sites were defined based on the published HB0801 sequence (GenBank accession number CP002058). The orientation of the mTn is indicated in parenthesis. b CDS are designated according to the nomenclature used in GenBank. c The relative position and orientation of the mTn are indicated for each CDS. d Mutants were designated according to transformation and clone numbers.