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Expression and purification of CHAPk in E. coli. (a) The schematic diagram of the pET28a-CHAPk expression vector. (b) SDS-PAGE analysis of the purified CHAPk. M: Protein ladder, lane 1: total protein of E.coli BL21-pET28a-CHAPk, lane 2: the peak of penetration, lane 3: the peak of elution. (c) MALDI-TOF MS analysis of the purified CHAPk.
