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. 2020 Jan 25;8(2):174. doi: 10.3390/microorganisms8020174

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knob formation of IT4 populations enriched for binding to HBEC-5i cells at 37 °C and 40 °C. Electron micrographs of erythrocytes infected with IT4^NE, IT4^CSAEC37°, IT4^CSAEC40°, and IT4^NE heat-shocked at 40 °C for 2 h weekly over a period of 5 weeks. Arrows indicate knobs.