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. 2020 Feb 8;8(2):226. doi: 10.3390/microorganisms8020226

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TLC analyses of secondary metabolites produced by strain H17 in presence (+Pb) or absence (-Pb) of lead. Boshnaloside (B) was used as reference standard. Retention front (Rf) of the compounds are indicated. (a) General derivatizers. (1) Visible light (Vis), (2) UV-254 nm, (3) UV-365 nm, (4) ferric chloride. (b) Detection of phenolics. (1) Sulphuric vainillin; (2) Borntrager-Vis; (3) Borntrager-365 nm; (4) modified Borntrager-Vis; (5) modified Borntrager-365 nm. (c) Detection of biogenic amines. (1) Draggendorff-Vis; (2) Ninhydrin; (3) Ninhydrin-365 nm; (4) Wagner-Vis; (5) Wagner-365 nm. Arrows (C1–C7) denote the bands that differentiate the chemical profile of both types of culture extracts.