Figure 7.

qPCR diagnosis of M. maydis and M. phaseolina in cotton sprouts. The full-growth season pot experiment under field conditions was conducted in the spring and summer of 2019. The charcoal-rot-susceptible cotton genotype Pima cotton, Goliath cv. was chosen for this experiment. The qPCR was used to identify the ability of the pathogens, alone or in mixed inoculation, to infect and colonize the cotton root tissues 37 days after sowing (DAS). The control is a soil taken from a nearby field with minor levels of M. maydis or M. phaseolina infestation. The y-axis values are M. maydis relative DNA (Mm) or M. phaseolina relative DNA (Mpk) abundance, normalized to the cytochrome c oxidase (Cox) DNA. Vertical upper bars represent the standard error of the mean of 10 replications (pots, each containing one plant).